As the global COVID-19 pandemic evolves, researchers worldwide are working hard to study the mechanisms underlying SARS-CoV-2 infection.
During virus infection, the respiratory tract epithelium forms a physical barrier that pathogens encounter. Understanding this barrier is greatly enhanced by having well characterized and standardized cell culture models of the epithelium. The ability to model the different properties and functions of the respiratory tract in vitro is key to successfully translating research findings into real-world medical applications. Our cells and cell culture supplier, PromoCell, support researchers in achieving this by providing well characterized and standardized human primary cells isolated from the proximal and distal respiratory tract. We also provide specialized cell culture media specialized for each primary cell type.
Using human primary airway epithelial cells for infection studies with SARS-CoV-2 allows researchers to study how the virus interacts with its natural host. his contrasts with studying the virus in cell lines, which are often derived from tumor tissue and display modified gene and protein expression patterns. Cell lines are therefore unable to give a true insight into how SARS-CoV-2 interacts with human tissue.
Products for Respiratory Research
Advantages of using Human Primary Cells
- A wide range of available donors give you access to primary cells from different genetic backgrounds and health status parameters (e.g. donors with COPD or asthma)
- Access to cells from various tissue localizations (nasal, tracheal, bronchial, small airways)
- A large stock inventory so you can plan multiple experiments using the same donor and media reagents
Selected publications featuring PromoCell products
Leading virology labs around the globe rely on PromoCell products for developing new strategies to fight COVID-19, and this work has already produced several publications.
A recent publication by Hoffmann et al. from the Pöhlmann lab at the German Primate Center in Göttingen, in cooperation with the Robert Koch Institute and the Drosten lab at Charité amongst others, has highlighted the mechanism of infection by SARS-CoV-2 through the SARS-CoV ACE2 host receptor and priming with TMPRSS2. This was investigated in primary bronchial epithelial cells isolated using PromoCell’s Airway Epithelial Basal Medium. Their studies suggest that antibodies targeted against SARS-CoV may offer protection against SARS-CoV-2.
A study by Lukassen et al. from the Eils lab at the Berlin Institute of Health, in cooperation with the group of Prof. Kreuter at the Heidelberg University Hospital amongst others, has revealed how tissue localization and patient-specific parameters influence host cell vulnerability. For this, the expression of SARS-CoV-2 host factors like ACE2, TMPRSS2 and FURIN in epithelial cells isolated from different patients and tissue localizations using PromoCell Airway Epithelial Growth Medium Supplements.
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