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COVID-19

COVID-19 Diagnostics

NEW SARS-CoV-2 Neutralizing Antibodies Detection Kit

AdipoGen Life Sciences has developed a sensitive, specific and fast SARS-CoV-2 Neutralizing Antibodies Detection Kit (Prod. No. AG-48B-0002) that can be used to detect the presence of neutralizing antibodies against SARS-CoV-2 in serum/plasma in about 2 houts independently of species and isotypes!.

This Detection Kit is based on a colorimetric reaction, which measures the binding of the RBD of the Spike S protein from SARS-CoV-2 to its human receptor ACE2. The presence of neutralizing/blocking antibodies in the samples are detected by reduction of signal indicating the inhibition of the Spike-ACE2 binding.

The SARS-CoV-2 Neutralizing Antibodies Detection Kit contains key reagents required to test the presence of functional neutralizing antibodies against SARS-CoV-2 present in the serum or plasma. It is an easy, fast and scalable (automation) alternative to the classical neutralization assays using Vero E6 cells, such as virus neutralization test (VNT) and pseudo-virus neutralization test (pVNT) and does not need biosafety requirements.

This kit would be administrable in vaccine and therapeutic development as it is suitable for all antibody isotypes and can be used to determine neutralizing antibodies in animal models. The kit will also be useful in current COVID-19 investigations of sero-prevalence, assessment of immune response, herd immunity, longevity of protective immunity, efficacy of different vaccine candidates as well as tracking infections in animals. 

NEW & UNIQUE  SARS-CoV-2 (Spike RBD) IgG Serological ELISA Kit

AdipoGen Life Sciences has developed a sensitive and specific SARS-CoV-2 (Spike RBD) IgG Serological ELISA Kit (Prod. No. AG-45B-0020) that can be used for the qualitative measurement of human Immunoglobulin G (IgG) against SARS-CoV-2 Spike (Receptor Binding Domain) protein in serum and plasma samples.

During an infection, IgM antibodies appear first, followed by IgA on mucosal surfaces or IgG in the serum. The spike (S) proteins (mainly the RBD domain) and the nucleocapsid (N) proteins are the main immunogens of coronaviruses leading to an antibody answer. This assay is an indirect ELISA assay (based on a coated Spike (RBD) protein).

Included in this assay and unique in the market for SARS-CoV-2 IgG assays, is a second plate (antigen non-coated Background Plate or False Positive Control Plate) to determine the sample specific background and to measure the amount of IgG antibodies non-specifically bound to the well. To measure presence of anti-SARS-CoV-2 human IgG antibodies in serum or plasma, net sample optical densities (Net OD) are calculated by subtracting each sample Background Plate OD from the Spike (SARS-CoV-2) antigen plate (Spike Plate) OD.

Note:

  • • In the first week of the onset of the infection with the novel coronavirus (SARS-CoV-2) patients results may be negative for IgG.
  • • Patients with low immunity or other diseases that affect immune function, failure of important systemic organs, and use of drugs that suppress immune function can also lead to negative results of new coronavirus IgG.
  • • Previous infection of SARS or other coronavirus strains may cause a light IgG positive in view of similarities in the nucleocapsid protein, but not in the Spike RBD protein. This means that nucleocapsid-based SARS-CoV-2 IgG assays have a higher chance of cross-reactivites with other coronavirus IgG antibodies than Spike RBD-based SARS-CoV-2 IgG assays, such as this newly developed assay from AdipoGen Life Sciences.
Biological Therapeutic Strategies against SARS-CoV-2 and COVID-19

SARS-CoV-2 Inhibitor Screening Set (Ref. AG-44B-0007) has developed a High Throughput Screening (HTS) detection assay for the determination of SARS-CoV-2 blocking reagents.

The SARS-CoV-2 Inhibitor Screening Kit contains key reagents required to facilitate identification of SARS-CoV-2 inhibitors. This inhibitor screen is based on a colorimetric ELISA kit, which measures the binding of the RBD of the Spike S protein from SARS-CoV-2 to its human receptor ACE2.

NEW ACE2 (human) ELISA Kit

AdipoGen Life Sciences has developed a sensitive and specific colorimeteric sandwich ACE2 (human) ELISA Kit (Prod. No. AG-45B-0023) that can to be used for the in vitro quantitative determination of soluble human ACE2 in cell culture supernatants, serum, plasma and urine.

ACE2 has been identified as a key receptor on target cells for SARS-CoV infections in 2002. ACE2 functions as the entry receptor of the new SARS-CoV-2 coronavirus that emerged in China in 2019 and is the cause of the new disease COVID-19. Strong binding of the spike protein of SARS-CoV-2 to ACE2, along with proteolytic cleavage of ACE2 by transmembrane serine protease 2 (TMPRSS2), facilitates entry of the virus into cells, viral replication and cell-to-cell transmission. ACE2 can undergo an ADAM17 (a disintegrin and metalloproteinase 17)-mediated “shedding” from endothelial cells, resulting in the release of the ectodomain into the circulation. This soluble form may act as a competitive interceptor of SARS-CoV-2 and other coronaviruses by preventing binding of the viral particle to the surface-bound, full-length ACE2. A reliable ELISA Kit to determine soluble ACE2 in the circulation can be useful in therapeutic developmental studies.

Soluble ACE2 might also be used as biomarker of hypertension and cardiovascular diseases.

Validated Recombinant Proteins for ACE2 & COVID-19 Research

Product Name Cat. No. Source Purity Application / Activity
ACE2 (human) (rec.) AG-40B-0192 HEK293 cells ≥95% (SDS-PAGE) Soluble human ACE2 competitively inhibits SARS-CoV-2 infection.
NEW ACE2 (human) (rec.) (Biotin) AG-40B-0192B HEK293 cells ≥95% (SDS-PAGE) Soluble human ACE2 competitively inhibits SARS-CoV-2 infection.
NEW ACE2 (human) (rec.) (His) CHI-B232008 HEK293 cells ≥90% (SDS-PAGE) Soluble human ACE2 competitively inhibits SARS-CoV-2 infection.
ACE2 (human):Fc (human) (rec.) CHI-B232006 HEK293 cells ≥90% (SDS-PAGE) Soluble human ACE2 competitively inhibits SARS-CoV-2 infection.
ACE2 (mouse) (rec.) AG-40B-0193 HEK293 cells ≥95% (SDS-PAGE) Soluble mouse ACE2 negative control.
SARS-CoV-2 Spike Protein S1 (RBD) (rec.) (His) CHI-B232004 HEK 293 cells ≥90% (SDS-PAGE) Soluble Protein S (RBD) competitively inhibits SARS-CoV-2 infection. For drug and antibody screening applications and immunization.
NEW SARS-CoV-2 Spike Protein S1 (RBD) (rec.) (GST-His) CHI-B249001 HEK 293 cells ≥95% (SDS-PAGE) Soluble Protein S (RBD) competitively inhibits SARS-CoV-2 infection. For drug and antibody screening applications and immunization.
SARS-CoV-2 Spike Protein S1 (RBD):Fc (human) (rec.)  CHI-B232003 HEK 293 cells ≥95% (SDS-PAGE) Soluble Protein S (RBD) competitively inhibits SARS-CoV-2 infection. For drug and antibody screening applications and immunization.
SARS-CoV-2 Spike Protein S1 (RBD):Fc (human) (rec.) AG-40B-0194 HEK 293 cells ≥95% (SDS-PAGE) Soluble Protein S (RBD) competitively inhibits SARS-CoV-2 infection. For drug and antibody screening applications and immunization.
SARS-CoV-2 Nucleocapsid Protein (rec.) (His) CHI-B233501 E. coli ≥95% (SDS-PAGE) For drug screening applications.
PLpro (SARS Coronavirus) (rec.) (His) SBB-DE0024 E. coli ≥95% (SDS-PAGE) Involved in the processing of the viral polyprotein.
ISG15 (human) (rec.) (Rhodamine 110) SBB-PS0002 E. coli ≥97% (LCMS) PLPro substrate. Inhibits viral budding and acts as IFNγ-inducing cytokine.

UNIQUE  Human ACE2 Monoclonal Blocking Antibody

AdipoGen Life Sciences’ anti-ACE2 (human), mAb (blocking) (AC384) (preservative free) (Prod. No. AG-20A-0037PF) is a monoclonal antibody that recognizes human ACE2 and works specifically in ELISA, Western Blot and Functional Application. The antibody blocks the binding of human ACE2 to the Spike protein of SARS-Cov-2.

Figure: Binding of ACE2 (human) to the Spike protein of SARS-CoV-2 is inhibited by the antibody anti-ACE2 (human), mAb (blocking) (AC384) (AG-20A-0037PF).

Method: SARS-CoV-2 Spike Protein S1 (RBD):Fc (human) (rec.) (AG-40B-0194) is coated on an ELISA plate at 1µg/ml. ACE2 (human), mAb (blocking) (AC384) (AG-20A-0037PF) or an unrelated mAb Control are added (starting at 40µg/ml with a two-fold serial dilution) together with 500ng/µl of ACE2 (human) (AG-40B-0192). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).

Flow Cytometry-Competent  Human ACE2 Monoclonal Antibodies 

AdipoGen Life Sciences’ anti-ACE2 (human), mAb (AC18F) (Prod. No. AG-20A-0032) is a monoclonal antibody clone that recognizes human ACE2 and works specifically in Flow Cytometry (FACS). The antibody is available in different formats, unlabeled (#AG-20A-0032) and Biotin-labeled (#AG-20A-0032B), as well as labeled with the dyes ATTO488 (#AG-20A-0032TD) and ATTO647N (#AG-20A-0032TS). All variants are FACS-competent using the appropriate secondary reagents.

Figure: Detection of endogenous human ACE2 by different formats of anti-ACE2 (human), mAb (AC18F) (AG-20B-0032).

Method: HepG2 cell line is stained with anti-ACE2 (human), mAb (AC18F) (red line) or an appropriate isotype control at 1µg/106 cells each, revealed with a secondary reagent and then analyzed by flow cytometry.

Download: Cell Detachment Protocol for FACS. 

Product Name Cat. No. Isotype Application Species
anti-ACE2 (human), mAb (AC18F) AG-20A-0032 Mouse IgG1κ ELISA, FACS, WB Human
anti-ACE2 (human), mAb (AC18F) (Biotin) AG-20A-0032B Mouse IgG1κ ELISA, FACS, WB Human
anti-ACE2 (human), mAb (AC18F) (ATTO 488) AG-20A-0032TD Mouse IgG1κ FACS Human
anti-ACE2 (human), mAb (AC18F) (ATTO 647N) AG-20A-0032TS Mouse IgG1κ FACS Human
anti-ACE2 (human), mAb (AC384) AG-20A-0037 Mouse IgG1κ ELISA, WB Human
anti-ACE2 (human), mAb (AC384) (Biotin) AG-20A-0037B Mouse IgG1κ ELISA, WB Human
anti-ACE2 (human), mAb (blocking) (AC384) (preservative free) AG-20A-0037PF Mouse IgG1κ FUNC (Blocking), ELISA, WB Human
anti-ACE2 (human), pAb AG-25A-0042 Rabbit ELISA, WB Human

Antiviral Compounds – Potential Small Molecule Therapeutics Against COVID-19

There are no approved drugs to treat the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection that causes coronavirus disease 2019 (COVID-19). Existing drugs, that have a known favorable safety profile, are being examined for strategies to treat the disease and fast-track a treatment plan. Several influenza and HIV drugs are currently undergoing clinical trial in coronavirus patients. The rational selection of drugs already on the market is being made based on their ability to inhibit any proteins essential for virus-receptor interaction and/or viral life cycle.

AdipoGen Life Sciences offers a Selection of Antiviral Small Molecules as Potential Tools for in vitro Studies of COVID-19 (not for human use). 

Product Name Cat. No. CAS Number Target Antiviral activity
NEW Aloxistatin [E-64d] AG-CR1-3737 88321-09-9 Viral entry
Cathepsin L
Viral Replication
Amastatin . hydrochloride AG-CP3-7003 100938-10-1 Viral entry
ANPEP (Aminopeptidase N)
Viral Replication
NEW Baricitinib AG-CR1-3734 1187594-09-7 JAK1/JAK2 / Immunomodulation
AP-2 associated protein kinase 1 / Endocytosis
Viral Replication
NEW Boceprevir AG-CR1-3735 394730-60-0 Main Protease (Mpro) Viral Transcription/Replication
Camostat mesylate AG-CR1-3716 59721-29-8 Viral entry
TMPRSS2
Viral Replication
Chloroquine . diphosphate AG-CR1-3721 50-63-5 Lysosome function
Zinc ionophore
Immunomodulation
Viral Replication
Hydroxychloroquine . sulfate AG-CR1-3720 747-36-4 Lysosome function
Zinc ionophore
Immunomodulation
Viral Replication
NEW Clevudine AG-CR1-3736 163252-36-6 RNA-dependent RNA polymerases (RdRps) Viral Transcription/Replication
Darunavir AG-CR1-3712 206361-99-1 Papain-like viral protease (PLVP) Viral Maturation/Replication
Darunavir . ethanolate AG-CR1-3724 635728-49-3 Papain-like viral protease (PLVP) Viral Maturation/Replication
NEW Dexamethasone AG-CR1-3742 50-02-2 Inflammatory Factors Cytokine Storm
Ebselen AG-CR1-0031 60940-34-3 Main Protease (Mpro)/3C-like Protease Viral Transcription/Replication
NEW EIDD-2801 AG-CR1-3733 2349386-89-4 RNA-dependent RNA polymerases (RdRps) Viral Transcription/Replication
Elbasvir AG-CR1-3729 1370468-36-2 RdRP, Papain-like Proteinase and Helicase Viral Replication
NEW Famotidine AG-CR1-3730 76824-35-6 SARS-CoV-2 papain-like protease (PLpro) Viral Replication
Favipiravir AG-CR1-3717 259793-96-9 RNA-dependent RNA polymerases (RdRps) Viral Transcription/Replication
NEW Ibudilast AG-CR1-3738 50847-11-5 Macrophage Inhibitory Factor (MIF)
Immunomodulation
Viral Replication
Cytokine Storm
Imatinib mesylate
AG-CR1-3725 220127-57-1 Virion fusion with endosomal membrane Viral Replication
Lopinavir AG-CR1-3715 192725-17-0 Coronavirus endopeptidase C30 (CEP_C30) Viral Maturation/Replication
Mycophenolic acid
AG-CN2-0419 24280-93-1 SARS-CoV-2 papain-like protease (PLpro) Viral Replication
NEW Nafamostat mesylate AG-CR1-3731 82956-11-4 Viral entry
TMPRSS2
Viral Replication
Nelfinavir . mesylate
AG-CR1-3726 159989-65-8 Post-entry inhibitor Viral Replication
Niclosamide
AG-CR1-3643 50-65-7 Endosome acidification Viral Replication
Niclosamide . ethanolamine
AG-CR1-3644 1420-04-8 Endosome acidification Viral Replication
Nitazoxanide
AG-CR1-3723 55981-09-4 Viral hemagglutinin
Viral IE2
Viral Maturation/
Transcription/Replication
Oseltamivir . phosphate
AG-CR1-3714 204255-11-8 Viral neuraminidase
Release of viral particles
Viral Replication
NEW PX-12 AG-CR1-3739 141400-58-0 Main Protease (Mpro)/3C-like Protease Viral Transcription/Replication
Remdesivir
AG-CR1-3713 1809249-37-3 RNA-dependent RNA polymerases (RdRps) Viral Transcription/Replication
Remdesivir Metabolite
GS-441524
AG-CR1-3722 1191237-69-0 RNA-dependent RNA polymerases (RdRps) Viral Transcription/Replication
Ribavirin
AG-CR1-3719 36791-04-5 RNA-dependent RNA polymerases (RdRps)
RNA capping activity
Viral mutation rates
Immunomodulation
Viral Transcription/Replication
Ritonavir
AG-CR1-3683 155213-67-5 Coronavirus endopeptidase C30 (CEP_C30) Viral Maturation/Replication
Rosuvastatin . calcium salt
AG-CR1-3728 147098-20-2 Main Protease Mpro Viral Replication
Ruxolitinib . phosphate salt
AG-CR1-3645 1092939-17-7 JAK1/JAK2
Immunomodulation
Viral Replication
Ruxolitinib (free base)
AG-CR1-3624 941678-49-5 JAK1/JAK2
Immunomodulation
Viral Replication
Saquinavir . mesylate
AG-CR1-3727 149845-06-7 Main Protease Mpro Viral Replication
Shikonin
AG-CN2-0487 517-89-5 Main Protease (Mpro)/3C-like Protease Viral Transcription/Replication
NEW Suramin . hexasodium salt AG-CR1-3575 129-46-4 Viral Entry/Fusion
RNA-dependent RNA polymerases (RdRps)
Viral Transcription/Replication
NEW TDZD-8 AG-CR1-3740 327036-89-5 Main Protease (Mpro)/3C-like Protease Viral Transcription/Replication
NEW Telaprevir AG-CR1-3741 402957-28-2 3C-like Protease Viral Replication
Tofacitinib
AG-CR1-3625 477600-75-2 JAK1/JAK3
Immunomodulation
Viral Replication
Tofacitinib citrate
CDX-T0461 540737-29-9 JAK1/JAK3
Immunomodulation
Viral Replication
Umifenovir . HCl [Arbidol]
AG-CR1-3718 131707-23-8 Viral entry
Fusion into host cells
Viral Replication

Cytokine Storm and Severity of COVID-19

The most critically ill COVID-19 patients are known to undergo a cytokine storm leading to poor prognosis and need of urgent anti-inflammatory treatment/hospitalization. There are many variations on this phenomenon and they go by many names: systemic inflammatory response syndrome, macrophage activation syndrome or cytokine release syndrome (CRS).

Therefore the diagnostic detection and treatment of cytokine storms has become an important part of rescuing severe patients. Targets like IL-1, IL-6, IL-7, IL-10, IL-18, IL-33, IFN-γ, TNF-α or many others might play an important role in cytokine release syndrome (CRS). Detection of their levels and blockage of their signaling pathways with immunomodulatory agents (biologicals, small molecules) is expected to become a new method for the treatment of severe patients.

AdipoGen Life Sciences offers a broad range of Cytokine Immunoassays as well as recombinant cytokines and blocking antibodies, which are already being successfully used to research the various cytokine storm factors involved and to characterize the immune response.

Target Product Name Cat. No.
Interleukin-6 Cymax IL-6 (human) ELISA Kit YIF-LF-EK0260
IL-6 (human):Fc (human) (rec.) CHI-HF-21006
Interleukin-1 Family / Inflammasome IL-1β (human) (rec.) (untagged) AG-40B-0023
Cymax IL-1β (human) ELISA Kit YIF-LF-EK0276
anti-NLRP3/NALP3, mAb (Cryo-2) AG-20B-0014
anti-Caspase-1 (p20) (human), mAb (Bally-1) AG-20B-0048
Interleukin-33 IL-33 (human) (rec.) (untagged) AG-40B-0038
IL-33 (oxidation resistant) (human) (rec.) (His) AG-40B-0167
IL-33 (oxidation resistant) (human) (rec.) (untagged) AG-40B-0160
anti-IL-33 (human), mAb (IL33305B) AG-20A-0041
anti-IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1) (PF) AG-27B-0013PF
IL-33 (human) ELISA Kit AG-45A-0033YEK
GM-CSF GM-CSF (human) (rec.) (His) CHI-HR-200CSF
Interleukin-2


IL-2 (human) (rec.) (untagged) CHI-HR-20602
IL-2 Superkine (Fc) AG-40B-0111
IL-2 Superkine (untagged) AG-40B-0187
TNF-α



TNF-α, Soluble (human) (rec.) AG-40B-0006
TNF-α (human) (multimeric) (rec.) AG-40B-0019
anti-TNF-α (human), mAb (J1D9) ANC-398-020
anti-TNF-α (mouse), mAb (blocking) (V1q) (PF) AG-20B-0081PF
Interleukin-10

IL-10 (human) (rec.) (untagged) CHI-HR-20610
IL-10 (human):Fc (human) (rec.) (non-lytic) CHI-HF-22010

 

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