CRISPR Genetic Screening

Genetic screening libraries using CRISPR are a widely used technique with which to identify gene targets that drive biological responses in cell lines or other model systems of interest.

• Identify essential genes for growth and proliferation.
• Find genes responsible for resistance or sensitivity to compounds or treatments.
• Look for lethal synthetic interactions with known genetic lesions.
• Elucidate the mechanism of action or target pathway for a compound or drug

Therefore, Cellecta creates customized CRISPR libraries for Knock-Out, inactivation or activation of certain genes, using sgRNA and shRNA combined with Cas9 and Cas9 modified for gene activation or inactivation.

These libraries targeting various genes are available as plasmids and/or already packaged, ready to be introduced via vectors. lentivirals.

Lentiviral Constructs

Lentiviral expression vectors are one of the most convenient and effective vehicles to stably introduce and express your gene of interest in almost any mammalian cell. Knockout, knockdown, or activate your target gene with CRISPR, CRISPRi, or CRISPRa sgRNA lentiviral constructs. With sgRNA, shRNA or cDNA.

Barcode customized libraries

Custom lentiviral barcode libraries that enable you to generate populations of millions of cells where each have their own uniquely identifiable barcode sequence. Barcodes permanently integrate into cells’ genomic DNA and are heritable by progeny Barcodes are expressed in transcripts so they can read directly from genomic DNA or by RNA sequencing.