Mabtech is a Swedish biotechnology company that develops immunoassays for life science research. Their goal is to aid research, by providing the scientific community with innovative tools, in particular for ELISpot, FluoroSpot, and ELISA. To that end, they develop and produce a wide range of monoclonal antibodies, kits, peptide pools, and instruments for in vitro applications.
The enzyme-linked immunospot (ELISpot) assay is an extremely sensitive immunoassay performed in a 96-well plate format to quantify protein secreting cells.
The assay principle is straight forward: cells are cultured inside a plate coated with capture antibody. Cytokines, immunoglobulins, or other target proteins secreted by the cells are captured immediately after secretion and throughout the stimulation process. After cell removal, secreted proteins are identified using a detection antibody. Visible spots form after adding a precipitating substrate. Each spot corresponds to an individual analyte-secreting cell.
FluoroSpot is the multiplex version of ELISpot. Just like in the ELISpot assay, target proteins secreted by cells are captured by specific antibodies immediately after secretion and throughout the stimulation process, making it an extremely sensitive sandwich assay. The difference to ELISpot lies in the mode of detection: where ELISpot relies on an enzymatic reaction, FluoroSpot utilizes fluorescence. This opens up for multiplex analyses of several analytes simultaneously, enabling studies of cell populations with different functional profiles.
Both readers have these common characteristics:
They differ in that one the Astor reads ELIspot and the Iris can read ELIspot and Fluorospot.
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