Universal Sequencing Technology (UST) was established by a group of NGS technology veterans from Roche/454, dedicated to the development of the most advanced cost-effective DNA sequencing technologies.

They developed a transposase based TELL-Seq linked read library prep kit technology, enabling Illumina sequencing platforms to produce super long-range results (average 20kb to 100kb).

The technology allows a scientist to do de novo sequencing (need for microbial researches, genomes of plants/animals/insects, etc.), and to identify structural variations among organisms and individuals more efficiently. Researchers will be able to phase genomes to distinguish parental origins of structural variants.

TELL-Seq™ Technology

Transposase Enzyme Linked Long-read Sequencing (TELL-Seq™) is a simple and scalable NGS library technology that generates barcode linked-reads for genome scale sequencing applications. The whole procedure can be carried out in a PCR tube without the need for expensive instrumentation. The TELL-Seq™ Whole Genome Sequencing (WGS) Library Prep Kit will generate an Illumina sequencing library in just 3 hours. The protocol can be easily adjusted based on the genome size to be analyzed. TELL-Seq será el nuevo método de biblioteca estándar para WGS.TELL-Seq will be the new standard library method for WGS.

Applications

How it Works?

DE NOVO ASSEMBLY

Using the TELL-Seq™ WGS library prep kit you can sequence novel genomes. This works for small bacterial genomes, medium sized insect, plant, and fungi genomes and large animal and plant genomes. The TELL-Seq™ kit will turn an ordinary short-read 2nd generation DNA sequencer into a long-read 3rd generation DNA sequencer.

METAGENOMICS SEQUENCING

Samples containing disparate microbes are essential to gaining insight into our ecosystem as well as human disease. The TELL-Seq™ WGS Library Prep kit will allow you to sequence samples at a metagenomic scale; providing long-read like results with the ease of short-read sequencing.

STRUCTURAL VARIATION

Short-read DNA sequencers have trouble identifying large structural variants. These could be in the form of copy number variants, translocations, duplications, deletions, insertions, and inversions. The TELL-Seq™ WGS Library Prep kit links short-reads turning them into long-reads. The linked, long-read data that you get using the TELL-Seq™ kit will allow you to take a step back and see what you were missing.

WHOLE GENOME PHASING

Knowing which homologous chromosome has which sequence is essential to understanding a genome. Whole Genome Phasing is difficult with 2nd generation, short-read, DNA sequencing technology. Phasing provides haplotype information which is important for understanding complex traits and variant linkages. The TELL-Seq™ WGS DNA Library Prep kit helps with this by linking short-reads to larger fragments and therefore long-reads.