riboPOOLs are an affordable and flexible solution for the depletion of ribosomal RNA (rRNA) or other custom RNA of any species. riboPOOLs is composed of highly complex clusters of optimized biotinylated DNA probes that allow efficient removal of abundant rRNA from total RNA samples isolated from prokaryotic or eukaryotic cells or tissues.
Benefits of riboPOOLs:
• Highly efficient and specific.
• rRNA depletion for any species
• Custom RNA depletion.
• Applicable to non-polyadenylated RNA.
Simple workflow with riboPOOLs
riboPOOLs consist of complex mixtures of 3′ biotinylated oligonucleotide probes. These probes are designed to specifically hybridize to all ribosomal RNAs of the target species. Ribosomal RNAs bound to riboPOOL probes are efficiently removed from samples by streptavidin-conjugated magnetic beads. RiboPOOLs work independently of RNA polyadenylation and can be applied for any subsequent application, e.g. ex. NGS library preparation.