oYo-Link® MNase Antibody Labeling Kit - Labels 500ug

oYo-Link® MNase allows for the site-specific labeling of All oYo-Link®-compatible antibodies with Micrococcal nuclease (MNase). MNase is derived from Staphylococcus aureus and is a relatively non-specific endonuclease that is capable of digesting single-stranded, double-stranded, circular and linear nucleic acids (DNA and RNA). MNase is widely used to study chromatin structure in chromatin immunoprecipitation (ChIP) assays by preferentially digesting naked DNA between nucleosomes and thus enriching the nucleosome- protected DNA fragments. The resulting undigested DNA is subjected to high-throughput sequencing and mapped to a reference genome. These assays can sometimes benefit from the direct conjugation of MNase to antibodies that target a desired transcription factor. Direct conjugation can also allow for epigenetic mapping within just a small number of cells, since the MNase can be directed to specific transcription factors or chromatin-associated proteins. One such approach is called the CUT&RUN Assay, whereby DNA is digested directly in cells using protein A (pA)-MNase or protein G (pG)-MNase conjugates. Since oYo-Link® MNase allows for the site-specific and covalent attachment of MNase directly to any antibody of choice, it can simplify in cell digestion assays by eliminating time-consuming incubation and wash-steps. It also prevents release of MNase. This can result in higher yield and higher resolution maps of genome-wide epigenetic changes. Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For additional questions concerning compatibility email support@alphathera.com