HiScript III RT SuperMix for qPCR (+gDNA wiper)

HiScript® III RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript® II Q RT SuperMix for qPCR (+gDNA wiper), including a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the optimal buffer optimized for reverse transcription further improve the efficiency of cDNA synthesis and are suitable for two-step qRT-PCR detection. The 4 × gDNA wiper Mix in the kit can completely remove the remaining genomic DNA in the RNA template, ensure that the subsequent quantitative results are more reliable, and simplify the design of qPCR primers without the need to design primers across introns; 5 × HiScript III qRT SuperMix contains All components required for reverse transcription reaction can be reacted quickly by adding template RNA and water, and at the same time, the gDNA wiper function is terminated to ensure the integrity of cDNA. The reverse transcription product is compatible with dye method and probe method qPCR, which can perform high-performance gene expression analysis. Application: Easy and quick operation: 5 × HiScript III qRT SuperMix contains all the components required for reverse transcription reaction, only need to add template RNA, the reverse transcription reaction can be completed within 20 minutes; Extensive template compatibility: Compatible with RNA templates of different species and poor integrity; Super impurity tolerance: It has super tolerance to common reverse transcription impurities such as ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid, etc.; Excellent reverse transcription efficiency: Compared with common commercial reverse transcription products, HiScript ® III RT SuperMix for qPCR (+gDNA wiper) reverses the smallest cDNA quantitative C T value and has excellent reverse transcription efficiency.