Category: Research kits

Reference: 781001-1
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The NOS Activity Assay measures NOS activity by monitoring the conversion of radiolabeled arginine to citrulline. This assay is simple, sensitive, and specific for NOS activity and can be used with both crude and...
Reference: 780051-2
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Cayman’s Nitrate/Nitrite Fluorometric Assay provides a convenient method for the quantitation of low levels of nitrate and nitrite in biological samples (particularly tissue culture medium). Previously undetectable...
Reference: 780001-2
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Cayman’s Nitrate/Nitrite Assay provides an accurate and convenient method for measurement of total nitrate/nitrite concentrations. This kit can be used to measure nitrate and nitrite in plasma/serum, urine, tissue...
Reference: 765021-96
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Arachidonoyl thio-PC is a substrate for cPLA2 by virtue of the presence of arachidonic acid at the sn-2 position of the glycerophospholipid. Hydrolysis of the arachidonoyl thioester bond at the sn-2 position by PLA2...
Reference: 765001-96
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Cayman’s sPLA2 Assay provides an accurate and convenient method for measurement of sPLA2 activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s...
Reference: 760901-96
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PAF-AH catalyzes the hydrolysis of the potent biologically-active phospholipid PAF, generating inactive lyso-PAF. Cayman’s PAF-AH assay kit provides an accurate and convenient method for measurement of PAF-AH...
Reference: 760871-96
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NADPH is an essential cofactor for the function of the NOS enzyme. Unfortunately, NADPH interferes with the chemistry of the Griess reagents, which are the most commonly used reagents for nitrite detection. This kit...
Reference: 760700-96
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This assay kit provides an accurate and convenient method for screening LO inhibitors. This assay measures the hydroperoxides generated from the incubation of a LO (5-, 12-, or 15-LO) with either arachidonic or...
Reference: 760151-96
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The COX Activity Assay utilizes the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized TMPD at 590 nm.The assay can be used to measure...
Reference: 760111-2
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The COX Colorimetric Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized TMPD at 590 nm....
Reference: 709001-96
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Cayman’s Antioxidant Assay measures the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysates. The assay relies on the ability of antioxidants in the sample to inhibit the oxidation of ABTS...
Reference: 707002-96
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Catalase is an ubiquitous antioxidant enzyme that is present in most aerobic cells. It is involved in the detoxification of H2O2, a ROS that is a toxic product of both normal aerobic metabolism and pathogenic ROS...
Reference: 707002-480
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Catalase is an ubiquitous antioxidant enzyme that is present in most aerobic cells. It is involved in the detoxification of H2O2, a ROS that is a toxic product of both normal aerobic metabolism and pathogenic ROS...
Reference: 706002-96
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Cayman’s SOD Assay is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates, and cell lysates. SOD activity is assessed by measuring the dismutation of superoxide...
Reference: 706002-480
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Cayman’s SOD Assay is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates, and cell lysates. SOD activity is assessed by measuring the dismutation of superoxide...
Reference: 705502-96
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Aconitase is an iron-sulfur protein containing a [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate. Whereas exposure of aconitase to oxidants renders the...
Reference: 705192-96
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MAGL is the main enzyme responsible for the inactivation of 2-AG. Cayman’s Monoacylglycerol Lipase Inhibitor Screening Assay provides a convenient method for screening human MAGL inhibitors. MAGL hydrolyzes...
Reference: 705003-96
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Cayman’s Lipid Hydroperoxide Assay measures the hydroperoxides directly utilizing the redox reactions with ferrous ions. An easy to use quantitative extraction method was developed to extract lipid hydroperoxides into...
Reference: 705002-100
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Quantification of lipid peroxidation is essential to assess the role of oxidative injury in pathophysiological disorders. Lipid peroxidation results in the formation of highly reactive, unstable hydroperoxides of both...
Reference: 704002-480
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Cayman’s Protein Determination is a microplate-based, colorimetric method for rapid total protein quantification. Based on the well-known Bradford method, it takes advantage of the color change of Coomassie® Dye when...
Reference: 703302-96
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GSTs are ubiquitous multifunctional enzymes, which play a key role in cellular detoxification. The enzymes protect cells against toxicants by conjugating them to glutathione thereby neutralizing their electrophilic...
Reference: 703202-96
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GR is a flavoprotein that catalyzes the NADPH-dependent reduction of oxidized GSSG to GSH. This enzyme is essential for the GSH redox cycle which maintains adequate levels of reduced cellular GSH. A high GSH/GSSG...
Reference: 703102-96
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GPX catalyzes the reduction of hydroperoxides, including H2O2, using reduced glutathione and thereby functions to protect the cell from oxidative damage. Cayman’s Glutathione Peroxidase Assay measures GPX activity...
Reference: 703102-480
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GPX catalyzes the reduction of hydroperoxides, including H2O2, using reduced glutathione and thereby functions to protect the cell from oxidative damage. Cayman’s Glutathione Peroxidase Assay measures GPX activity...
Reference: 703002-96
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Cayman’s GSH Assay utilizes a carefully optimized enzymatic recycling method for the quantification of GSH in a 96-well microplate format. It measures both GSH and GSSG to reflect total glutathione in a sample. The...
Reference: 703002-480
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Cayman’s GSH Assay utilizes a carefully optimized enzymatic recycling method for the quantification of GSH in a 96-well microplate format. It measures both GSH and GSSG to reflect total glutathione in a sample. The...
Reference: 701900-96
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Cayman's FSP1 Fluorescent Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human FSP1, a negative regulator of the ferroptosis pathway. The assay measures...
Reference: 701900-384
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Cayman's FSP1 Fluorescent Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human FSP1, a negative regulator of the ferroptosis pathway. The assay measures...
Reference: 701890-384
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Cayman’s Caspase-6 Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human caspase-6, a cysteine protease implicated in multiple neurodegenerative disorders....
Reference: 701880-96
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Cayman's GPX4 Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human GPX4, a negative regulator of the ferroptosis pathway. The assay measures GPX4...
Reference: 701840-384
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Cayman’s Caspase-1 Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human caspase-1, the major downstream effector following inflammasome activation. The...
Reference: 701820-384
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Cayman’s Caspase-4 Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human caspase-4, a major upstream effector of non-canonical inflammasome activation. The...
Reference: 701780-480
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Cayman’s Protein Determination (BCA) Kit is a quantitative assay that can be used with various buffers, water, plasma, serum, urine, and cell lysates. The assay has a dynamic range of 25 to 2,000 µg/ml and a lower...
Reference: 701760-96
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Cayman’s Protein Aggregation Assay Kit provides a fast and convenient method to determine the level of protein aggregation in a purified antibody solution or other protein solutions after generating an appropriate...
Reference: 701710-480
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Cayman’s Alkaline Phosphatase (ALP) Colorimetric Activity Assay Kit provides a convenient method of measuring ALP activity in serum, plasma, tissue samples, and cell lysates with a limit of detection of 0.5 U/L....
Reference: 701640-96
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Cayman’s Aspartate Aminotransferase Colorimetric Activity Assay Kit provides a convenient method of detecting AST activity in serum, plasma, tissue samples, and cell lysates. Measurement of the AST activity is carried...
Reference: 701600-96
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As one of the major sources of reactive oxygen species (ROS) in the cell, mitochondrial ROS has often been difficult to single out from the other sources of ROS within the cell. While some ROS dyes such as DHE and...
Reference: 701560-500
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Cayman’s JC-1 Mitochondrial Membrane Potential Flow Cytometry Assay Kit can be used to study mitochondrial behavior in a variety of conditions, including apoptosis. Changes in ΔψM, reflected by aggregation level of...
Reference: 701530-96
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Cayman’s Protein Carbonyl Fluorometric Assay Kit utilizes the rhodamine B hydrazide (RBH) reaction to measure the protein carbonyl content in plasma, serum, cell lysates, or tissue homogenates in a convenient 96-well...
Reference: 701490-96
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Cayman’s DUB Activity Assay Kit facilitates the rapid, robust measurement of deubiquitinating enzyme activity in vitro. The kit utilizes a high purity, fluorogenic substrate (ubiquitin-AMC) together with suitable...
Reference: 701470-96
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Cayman’s PAD3 Inhibitor Screening Assay Kit (Ammonia) provides a convenient method for screening human PAD3 inhibitors. PAD3 deiminates N-α-benzoyl-L-arginine ethyl ester (BAEE), a non-natural substrate with similar...
Reference: 701450-96
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Cayman’s PAD1 Inhibitor Screening Assay Kit (Ammonia) provides a convenient method for screening human PAD1 inhibitors. PAD1 deiminates N-α-benzoyl-L-arginine ethyl ester (BAEE), a non-natural substrate with similar...
Reference: 701440-96
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Cayman’s PAD1 Inhibitor Screening Assay Kit (AMC) provides a convenient method for screening human PAD1 inhibitors. This assay utilizes a fluorescent substrate (Z-Arg-AMC) consisting of an arginine residue, a...
Reference: 701410-96
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Cayman’s Protease Activity Assay Kit provides a convenient method for determining the activity of proteases in samples. Proteolytic digestion of the FITC-casein substrate can be monitored by changes in either...
Reference: 701390-96
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PAD2 is a guanidino-modifying enzyme that catalyzes the conversion of specific arginine residues to citrulline in a calcium-dependent manner. This enzyme has been implicated in several diseases, including rheumatoid...
Reference: 701350-96
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Cayman’s α-KG Fluorometric Detection Assay provides a fluorescence-based method for quantifying α-KG in biological samples such as serum, plasma, urine, and tissue. It can also be utilized to determine intracellular...
Reference: 701320-96
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PAD4 is a guanidino-modifying enzyme that functions as a transcriptional coregulator catalyzing the conversion of specific arginine residues to citrulline. Substrates for PAD4 include histones H2A, H3, and H4. PAD4...
Reference: 701310-500
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Cayman’s TMRE Mitochondrial Membrane Potential Assay Kit utilizes tetramethylrhodamine ethyl ester (TMRE), a cell permeable, cationic dye which accumulates in the mitochondrial matrix based on mitochondrial membrane...
Reference: 701230-96
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The COX (human) Inhibitor Screening Assay Kit directly measures PGF2α by SnCl2 reduction of COX-derived PGH2 produced in the COX reaction. The prostanoid product is quantified via ELISA using a broadly specific...
Reference: 701220-2
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Cayman's Calcium Assay provides a quick, reliable method for determining total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates. The assay utilizes an optimized...
Reference: 701090-96
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COX-1 and COX-2 catalyze the production of the PG and TX precursor PGH2. COX-2 is primarily responsible for the biosynthesis of PGs under acute inflammatory conditions. The COX-1 (ovine) Inhibitor Screening Assay...
Reference: 701080-96
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COX-1 and COX-2 catalyze the production of the PG and TX precursor PGH2. COX-2 is primarily responsible for the biosynthesis of PGs under acute inflammatory conditions. The COX-2 (human recombinant) Inhibitor...
Reference: 701040-96
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The condensation of the dicarboxylate oxaloacetate and acetyl CoA to the tricarboxylate citrate is catalyzed by citrate synthase. It is within this reaction that carbon molecules (as acetyl CoA) obtained from pyruvate...
Reference: 701010-1
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When measuring mitochondrial function or the effects of an unknown on mitochondrial function, it can be difficult to determine a mechanism using whole organisms or tissue. When this is the case, isolated mitochondria...
Reference: 701000-96
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The mitochondrial F1FO ATP synthase, commonly referred to as complex V, is a multi-subunit protein that utilizes energy in the form of a proton gradient generated by the electron transport chain, to phosphorylate ADP...
Reference: 700990-96
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Complex IV (cytochrome c oxidase) is the terminal electron acceptor in the mitochondrial electron transport chain. Complex IV functions by oxidizing cytochrome c and completing a four-electron reduction of oxygen (O2)...
Reference: 700950-96
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Complex III (CoQ cytochrome c oxidoreductase) is an essential protein for mitochondrial oxidative phosphorylation. Complex III functions as both a gatekeeper for mitochondrial respiration and as a major source of...
Reference: 700940-96
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Complex II (succinate dehydrogenase/co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex II catalyzes the oxidation of succinate to...
Reference: 700930-96
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Complex I (NADH oxidase/Co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex I catalyzes the 2 electron oxidation of NADH followed by the...
Reference: 700870-96
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Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from PUFAs, are unstable...
Reference: 700800-96
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Aldehyde dehydrogenases (ALDHs) represent a group of enzymes that oxidize a wide range of endogenous and exogenous aldehydes to their corresponding carboxylic acids, which affect both developmental and toxicological...
Reference: 700750-96
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Glucose-6-phosphate (G6P) plays a key role in the homeostatic regulation of blood glucose levels by participating in glycolysis, glycogen synthesis, gluconeogenesis, and can be metabolized to NADPH, enabling...
Reference: 700740-96
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β-Hydroxybutyrate (β-HB; 3-hydroxybutyric acid) is produced in the liver, mainly from the oxidation of fatty acids, and exported to peripheral tissues for use as an energy source. Measurement of β-HB can provide a...
Reference: 700640-96
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Lipases perform essential roles in the digestion, transportation, and processing of dietary lipids by controlling the clearance of triglyceride-rich lipoproteins from the circulation. Manipulating lipolysis has...
Reference: 700620-96
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Urea is the final degradation product of protein and amino acid metabolism. Urea is found in blood and is excreted by the kidney as a component of urine. Besides its role as carrier of waste nitrogen, urea also plays...
Reference: 700580-96
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Autotaxin (ATX, Ectonucleotide Pyrophosphatase/Phosphodiesterase-2, ENPP-2, Lysophospholipase D) is a secreted lysophospholipase D that catalyzes the hydrolysis of lysophosphatidylcholine (LPC) to generate...
Reference: 700560-96
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Protein Arginine Deiminase 4 (PAD4) is a guanidino-modifying enzyme that functions as a transcriptional coregulator catalyzing the conversion of specific arginine residues to citrulline. Substrates for PAD4 include...
Reference: 700540-2
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Hemoglobin (Hb or Hgb) is a globular protein found primarily in erythrocytes that carries oxygen from the lungs to tissue where it releases oxygen and then returns carbon dioxide (CO2) from the tissue to the lungs....
Reference: 700520-96
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D(−)-Lactate is a stereoisomer of lactate and is present in blood at only 1-5% the concentration of L(+)-lactate. Exogenous sources of D-lactate include fermented foods such as yogurt, sauerkraut, and pickles....
Reference: 700510-96
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L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance between NAD+ and NADH, which is...
Reference: 700480-96
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Glycogen is the principal storage form of glucose in animal and human cells. It is made primarily by the liver and muscles, but it can also be made by glycogenesis within the brain and stomach. Cayman’s Glycogen Assay...
Reference: 700470-96
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Pyruvate (pyruvic acid) is a key intermediate in cellular metabolic pathways and is derived primarily from glucose via glycolysis. Abnormal blood pyruvate levels are reported in a number of disorders including shock,...
Reference: 700460-2
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Creatinine is commonly measured in urine and is a key benchmark for the normalization of a variety of urinary biomarkers. Serum creatinine levels, however, are a useful indicator of renal function and abnormal levels...
Reference: 700420-96
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Ascorbate (L-Ascorbic acid or Vitamin C) is a six-carbon lactone that is synthesized from glucose in the liver of most mammalian species, but not by humans who must obtain ascorbate through dietary consumption. In...
Reference: 700340-96
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The detection and measurement of free thiols (i.e., free cysteine, glutathione, and cysteine residues on proteins) is one of the essential tasks for investigating biological processes and events in many biological...
Reference: 700340-480
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The detection and measurement of free thiols (i.e., free cysteine, glutathione, and cysteine residues on proteins) is one of the essential tasks for investigating biological processes and events in many biological...
Reference: 700320-96
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Uric acid (urate) is the end product of human purine metabolism and is mainly excreted in urine. Many factors, including genetic components and acquired factors, such as obesity and alcohol consumption, influence...
Reference: 700310-96
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The measurement of FFA can be useful in determining metabolic status. Cayman’s Free Fatty Acid (FFA) Assay can be used for measuring free fatty acids in plasma, serum, and urine. The FFA Assay utilizes a coupled...
Reference: 700300-96
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Glucose-6-phosphate dehydrogenase (G6PDH) is a cytosolic enzyme that catalyzes the first step in the pentose phosphate pathway. This pathway includes converting glucose to ribose-5-phosphate, a precursor to RNA, DNA,...
Reference: 700280-96
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The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) that catalyze a reaction coupling lysine deacetylation to the formation of nicotinamide and...
Reference: 700260-96
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ALT is a homodimeric cytoplasmic pyridoxal phosphate-dependent enzyme involved in cellular nitrogen metabolism, amino acid metabolism, and liver gluconeogenesis. Cayman’s Alanine Transaminase Assay provides a...
Reference: 700230-96
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Human HDAC8 is a class I HDAC and has been identified in a variety of human cancer tissues. Cayman’s HDAC8 Inhibitor Screening Assay provides a convenient fluorescence-based method for screening HDAC8 inhibitors. The...
Reference: 700210-96
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DPP (IV) inhibitors have emerged as a new class of oral antidiabetic agents. These inhibitors promote glucose homeostasis by inhibiting degradation of glucose-dependent insulinotropic polypeptide (GIP) and...
Reference: 700200-2
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Cayman’s COX Fluorescent Activity Assay provides a convenient fluorescence-based method for detecting COX-1 or COX-2 activity in both crude (cell lysates/tissue homogenates) and purified enzyme preparations. The assay...
Reference: 700190-96
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β-HB is a "ketone body" which is produced in the liver, mainly from the oxidation of fatty acids, and is exported to peripheral tissues for use as an energy source. Normally ketosis can indicate that lipid metabolism...
Reference: 700170-2
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Cayman’s MPO Inhibitor Screening Assay provides convenient fluorescence-based methods for screening inhibitors to both the chlorination and peroxidation activities of MPO. Sufficient reagents are provided for a full...
Reference: 700160-2
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Cayman’s MPO Peroxidation Fluorometric Assay provides a convenient fluorescence-based method for detecting MPO peroxidase activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the...
Reference: 700150-96
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Cayman’s MT Fluorometric Assay is a continuous enzyme-coupled assay that can continuously monitor SAM-dependent MTs. The removal of the methyl group from SAM generates AdoHcy, which is rapidly converted to urate and...
Reference: 700140-96
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Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing, and chromatin regulation. The SAM...
Reference: 700120-96
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LSD1 is a histone demethylase whose actions on specific lysine residues alter transcription of chromosomal DNA. It also inhibits the tumor suppressor activity of p53 by demethylating a specific lysine residue....
Reference: 700100-2
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Cayman’s COX Fluorescent Inhibitor Screening Assay provides a convenient fluorescence-based method for screening ovine COX-1 and human recombinant COX-2 for isozyme-specific inhibitors. The assay utilizes the...
Reference: 660990-24
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Apoptosis is associated with the fragmentation of chromosomal DNA into multiples of the 180 bp nucleosomal unit, known as DNA laddering. This kit is designed to maximize extraction of small fragments of DNA with...
Reference: 601950-96
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Cayman’s STAT3 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific STAT3 DNA binding activity in nuclear extracts. As an alternative to the cumbersome electrophoretic mobility...
Reference: 601930-1
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Cayman's Carbamylation IP Kit allows for the capture and pulldown of carbamylated proteins from cell lysates using a sorbent coupled with an anti-carbamylation monoclonal antibody. The carbamylated proteins can be...
Reference: 601810-96
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Lysosomes are intracellular organelles that contain enzymes used for the hydrolysis of waste materials and other cellular debris. The pH of the lysosome is ~4.5-4.8 and is optimal for these hydrolytic enzymes. The...
Reference: 601800-1
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Cayman’s Prostaglandin F Receptor Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for expressing the FP receptor and a CREB-regulated SEAP reporter (FP...

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