Category: Research kits

Reference: 600450-1
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Cayman’s Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting L-lactate, the end product of glycolysis, produced and secreted by cultured cells. In this assay, lactate dehydrogenase catalyzes...
Reference: 600470-1
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Glucose is stored in the muscles and liver as glycogen which is hydrolyzed to glucose and released into the blood when needed. The rate of glucose uptake in cells is dynamic and tightly regulated by hormones and/or...
Reference: 600490-1920
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Most histone lysine methyltransferases contain a conserved domain (SET) that utilizes S-adenosyl-L-methionine (SAM or AdoMet) as a co-factor to catalyze the methylation of lysine. SET7/9 (KMT7) is a SET...
Reference: 600490-384
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Most histone lysine methyltransferases contain a conserved domain (SET) that utilizes S-adenosyl-L-methionine (SAM or AdoMet) as a co-factor to catalyze the methylation of lysine. SET7/9 (KMT7) is a SET...
Reference: 600540-1
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Cayman’s Phagocytosis Assay Kit (IgG PE) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be...
Reference: 600570-1920
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Most histone lysine methyltransferases contain a conserved domain (SET) that utilizes S-adenosyl-L-methionine (SAM or AdoMet) as a co-factor to catalyze the methylation of the epsilon amino group of lysine. G9a-like...
Reference: 600570-384
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Most histone lysine methyltransferases contain a conserved domain (SET) that utilizes S-adenosyl-L-methionine (SAM or AdoMet) as a co-factor to catalyze the methylation of the epsilon amino group of lysine. G9a-like...
Reference: 600580-1920
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Most histone lysine methyltransferases contain a conserved domain (SET) that utilizes S-adenosyl-L-methionine (SAM or AdoMet) as a co-factor to catalyze the methylation of the epsilon amino group of lysine....
Reference: 600580-384
€0.00 (tax incl.)
Most histone lysine methyltransferases contain a conserved domain (SET) that utilizes S-adenosyl-L-methionine (SAM or AdoMet) as a co-factor to catalyze the methylation of the epsilon amino group of lysine....
Reference: 600590-96
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Nuclear factor E2-related factor 2 (Nrf2) is a transcription factor that plays a key role in maintaining redox homeostasis via its interaction with a cysteine-rich protein Kelch-like ECH-associated protein 1 (Keap1)....
Reference: 600610-2
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Neutrophil elastase is stored within cytoplasmic azurophilic granules in the neutrophil and released upon stimulation by pathogens where it acts either as free protein or is associated with networks of extracellular...
Reference: 600616-384
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Cayman’s PPARγ-LBD Ligand Screening Assay Kit provides a convenient fluorescence polarization (FP)-based single-step assay for screening ligands of the PPARγ-Ligand Binding Domain (PPARγ-LBD). In this assay, a ligand...
Reference: 600620-2
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Myeloperoxidase (MPO), released from neutrophils to degrade invading pathogens, provides one of the earliest lines of defense in innate immunity. It catalyzes the hydrogen peroxide-mediated oxidation of halide ions to...
Reference: 600670-100
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Early screening of a drug candidate’s potential to induce or inhibit CYP1A1/2 is a critical step in the drug development process. Cayman's CYP1A1/2 Induction Reporter Assay Kit is a novel method to assess CYP1A2...
Reference: 600680-100
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Early screening of a drug candidate’s potential to induce or inhibit CYP2B6 is a critical step in the drug development process. Cayman's CYP2B6 Induction Reporter Assay Kit is a novel method to evaluate human...
Reference: 600800-96
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The oxygen consumption rate (OCR) of cells is an important indicator of normal cellular function. It is used as a parameter to study mitochondrial function as well as a marker of factors triggering the switch from...
Reference: 601010-1
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Cayman's NETosis Assay Kit provides a simple and fast method for studying the process of NETosis ex vivo. Notably, Cayman's NETosis Assay Kit does not depend upon the DNA component of neutrophil extracellular traps...
Reference: 601080-96
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Oct4 is an essential regulator of embryonic stem cell pluripotency and used as a marker for undifferentiated cells. It also plays a role in gene expression, DNA repair and/or replication, signal transduction and...
Reference: 601100-96
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Cayman’s Protein Synthesis Assay Kit is a non-radioactive method for measuring protein synthesis that employs the cell-permeable, alkyne-containing, puromycin analog O-Propargyl-puromycin (OPP). Upon application to...
Reference: 601120-100
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Early screening of drug candidate’s potential to induce or inhibit CYP2C9 is a critical step in the drug development process. The CYP2C9 Induction Reporter Assay Kit is a novel method to evaluate CYP2C9 induction by...
Reference: 601180-1
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The oxidation of low density lipoprotein is thought to be one of the first steps in atherosclerosis. The uptake of oxidized LDL (oxLDL) by macrophages activates pro-inflammatory mediators that promote disease...
Reference: 601190-1
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FFAR1/GPR40 is a G protein-coupled receptor for long-chain fatty acids such as DHA and EPA ω-3 fatty acids. It is involved in the regulation of insulin release from pancreatic β cells and mediates triglyceride-induced...
Reference: 601200-1
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FFAR4/GPR120 is a G protein-coupled receptor for long chain fatty acids such as DHA and EPA ω-3 fatty acids. It is involved in the regulation of glucose uptake in adipocytes and mediates anti-inflammatory activity in...
Reference: 60122-5
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Source: Active recombinant N-terminal His-tagged COX-2N580A mutant purified from insect cells • Amino acids: 20-604 • MW: 67.9 kDa
Reference: 601280-100
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Cayman's Multi-Parameter Apoptosis Assay Kit is a simple way to probe for multiple different apoptotic readouts at the same time. This kit employs FITC-conjugated Annexin V as a probe for phosphatidylserine on the...
Reference: 601290-96
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Dihydroethidium (hydroethidine or DHE) is a widely used ethidium-based, redox-sensitive fluorescent probe. DHE has been shown to be oxidized by superoxide to form 2-hydroxyethidium (2-OH-E+) (ex 500-530 nm/em 590-620...
Reference: 601360-100
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Cayman's Early Apoptosis Detection Assay Kit employs FITC-conjugated annexin V as a probe for phosphatidylserine (PS) on the outer membrane of apoptotic cells, TMRE as a probe for ΔΨm, DAPI as an indicator of membrane...
Reference: 601370-100
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Cayman’s E. coli Phagocytosis Assay Kit employs FITC-labeled, heat-inactivated E. coli cells for the measurement of the phagocytic process in vitro. The engulfed fluorescent E. coli can be detected by flow cytometry.
Reference: 601380-96
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PGE2 is one of the most important biologically active prostanoids which exerts its actions mainly by binding to four distinct E-type prostanoid receptors: EP1, EP2, EP3, and EP4. These four GPCRs exhibit differences...
Reference: 601390-96
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PGE2 is one of the most important biologically active prostanoids which exerts its actions mainly by binding to four distinct E-type prostanoid receptors: EP1, EP2, EP3, and EP4. These four GPCRs exhibit differences...
Reference: 601410-100
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Cayman's Annexin V APC Assay Kit employs an APC-conjugated annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells. DAPI is used as a marker of cell membrane permeability seen in very late...
Reference: 601420-100
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Cayman's Annexin V PE Assay Kit employs a PE-conjugated annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells. DAPI is used as a marker of cell membrane permeability seen in very late...
Reference: 601440-96
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Cayman's TGR5 (GP-BAR1) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for expressing TGR5 and a cAMP response element regulated secreted alkaline...
Reference: 601470-48
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Cayman’s LDL Uptake Flow Cytometry Assay Kit employs human LDL conjugated to DyLight™ 488 as a convenient tool for studying the uptake of LDL in cultured cells. Flow cytometry provides the advantage of assessing the...
Reference: 601480-1
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Cayman’s Phagocytosis Assay Kit (IgG-DyLight™ 405) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can...
Reference: 601490-1
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Cayman’s Phagocytosis Assay Kit (IgG-DyLight™ 633) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can...
Reference: 601520-96
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Cayman’s ROS Detection Cell-Based Assay Kit (DCFDA) uses DCFDA as a fluorescent probe for the detection of ROS generation. Pyocyanin, a cell-permeable compound capable of redox cycling, is included as a positive...
Reference: 601740-1
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Macrophages are long-lived tissue resident cells that function to ingest and kill invading pathogens and to direct the ensuing immune responses. Cayman’s Macrophage (mouse) Elicitation Kit provides the reagents...
Reference: 601750-96
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,Cayman’s NETosis Imaging Kit provides a simple staining protocol for visualizing the process of NETosis kinetically ex vivo and in vitro. A cell-permeable DNA dye allows visualization of the dynamic changes of the...
Reference: 601770-1
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Cayman’s Efferocytosis Assay Kit employs CytoTell™ Blue to visualize effector (phagocytic) cells, CFSE to visualize bait (apoptotic) cells and staurosporine, a protein kinase C inhibitor, to induce apoptosis. The...
Reference: 601790-1
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Cayman’s Bile Acid Nuclear Receptor FXR (NR1H4) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for expressing FXR and a BSEP promoter-regulated SEAP...
Reference: 601800-1
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Cayman’s Prostaglandin F Receptor Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for expressing the FP receptor and a CREB-regulated SEAP reporter (FP...
Reference: 601810-96
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Lysosomes are intracellular organelles that contain enzymes used for the hydrolysis of waste materials and other cellular debris. The pH of the lysosome is ~4.5-4.8 and is optimal for these hydrolytic enzymes. The...
Reference: 601930-1
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Cayman's Carbamylation IP Kit allows for the capture and pulldown of carbamylated proteins from cell lysates using a sorbent coupled with an anti-carbamylation monoclonal antibody. The carbamylated proteins can be...
Reference: 601950-96
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Cayman’s STAT3 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific STAT3 DNA binding activity in nuclear extracts. As an alternative to the cumbersome electrophoretic mobility...
Reference: 660990-24
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Apoptosis is associated with the fragmentation of chromosomal DNA into multiples of the 180 bp nucleosomal unit, known as DNA laddering. This kit is designed to maximize extraction of small fragments of DNA with...
Reference: 700100-2
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Cayman’s COX Fluorescent Inhibitor Screening Assay provides a convenient fluorescence-based method for screening ovine COX-1 and human recombinant COX-2 for isozyme-specific inhibitors. The assay utilizes the...
Reference: 700120-96
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LSD1 is a histone demethylase whose actions on specific lysine residues alter transcription of chromosomal DNA. It also inhibits the tumor suppressor activity of p53 by demethylating a specific lysine residue....
Reference: 700140-96
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Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing, and chromatin regulation. The SAM...
Reference: 700150-96
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Cayman’s MT Fluorometric Assay is a continuous enzyme-coupled assay that can continuously monitor SAM-dependent MTs. The removal of the methyl group from SAM generates AdoHcy, which is rapidly converted to urate and...
Reference: 700160-2
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Cayman’s MPO Peroxidation Fluorometric Assay provides a convenient fluorescence-based method for detecting MPO peroxidase activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the...
Reference: 700170-2
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Cayman’s MPO Inhibitor Screening Assay provides convenient fluorescence-based methods for screening inhibitors to both the chlorination and peroxidation activities of MPO. Sufficient reagents are provided for a full...
Reference: 700190-96
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β-HB is a "ketone body" which is produced in the liver, mainly from the oxidation of fatty acids, and is exported to peripheral tissues for use as an energy source. Normally ketosis can indicate that lipid metabolism...
Reference: 700200-2
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Cayman’s COX Fluorescent Activity Assay provides a convenient fluorescence-based method for detecting COX-1 or COX-2 activity in both crude (cell lysates/tissue homogenates) and purified enzyme preparations. The assay...
Reference: 700210-96
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DPP (IV) inhibitors have emerged as a new class of oral antidiabetic agents. These inhibitors promote glucose homeostasis by inhibiting degradation of glucose-dependent insulinotropic polypeptide (GIP) and...
Reference: 700230-96
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Human HDAC8 is a class I HDAC and has been identified in a variety of human cancer tissues. Cayman’s HDAC8 Inhibitor Screening Assay provides a convenient fluorescence-based method for screening HDAC8 inhibitors. The...
Reference: 700260-96
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ALT is a homodimeric cytoplasmic pyridoxal phosphate-dependent enzyme involved in cellular nitrogen metabolism, amino acid metabolism, and liver gluconeogenesis. Cayman’s Alanine Transaminase Assay provides a...
Reference: 700280-96
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The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) that catalyze a reaction coupling lysine deacetylation to the formation of nicotinamide and...
Reference: 700300-96
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Glucose-6-phosphate dehydrogenase (G6PDH) is a cytosolic enzyme that catalyzes the first step in the pentose phosphate pathway. This pathway includes converting glucose to ribose-5-phosphate, a precursor to RNA, DNA,...
Reference: 700310-96
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The measurement of FFA can be useful in determining metabolic status. Cayman’s Free Fatty Acid (FFA) Assay can be used for measuring free fatty acids in plasma, serum, and urine. The FFA Assay utilizes a coupled...
Reference: 700320-96
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Uric acid (urate) is the end product of human purine metabolism and is mainly excreted in urine. Many factors, including genetic components and acquired factors, such as obesity and alcohol consumption, influence...
Reference: 700340-480
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The detection and measurement of free thiols (i.e., free cysteine, glutathione, and cysteine residues on proteins) is one of the essential tasks for investigating biological processes and events in many biological...
Reference: 700340-96
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The detection and measurement of free thiols (i.e., free cysteine, glutathione, and cysteine residues on proteins) is one of the essential tasks for investigating biological processes and events in many biological...
Reference: 700420-96
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Ascorbate (L-Ascorbic acid or Vitamin C) is a six-carbon lactone that is synthesized from glucose in the liver of most mammalian species, but not by humans who must obtain ascorbate through dietary consumption. In...
Reference: 700460-2
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Creatinine is commonly measured in urine and is a key benchmark for the normalization of a variety of urinary biomarkers. Serum creatinine levels, however, are a useful indicator of renal function and abnormal levels...
Reference: 700470-96
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Pyruvate (pyruvic acid) is a key intermediate in cellular metabolic pathways and is derived primarily from glucose via glycolysis. Abnormal blood pyruvate levels are reported in a number of disorders including shock,...
Reference: 700480-96
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Glycogen is the principal storage form of glucose in animal and human cells. It is made primarily by the liver and muscles, but it can also be made by glycogenesis within the brain and stomach. Cayman’s Glycogen Assay...
Reference: 700510-96
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L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance between NAD+ and NADH, which is...
Reference: 700520-96
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D(−)-Lactate is a stereoisomer of lactate and is present in blood at only 1-5% the concentration of L(+)-lactate. Exogenous sources of D-lactate include fermented foods such as yogurt, sauerkraut, and pickles....
Reference: 700540-2
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Hemoglobin (Hb or Hgb) is a globular protein found primarily in erythrocytes that carries oxygen from the lungs to tissue where it releases oxygen and then returns carbon dioxide (CO2) from the tissue to the lungs....
Reference: 700560-96
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Protein Arginine Deiminase 4 (PAD4) is a guanidino-modifying enzyme that functions as a transcriptional coregulator catalyzing the conversion of specific arginine residues to citrulline. Substrates for PAD4 include...
Reference: 700580-96
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Autotaxin (ATX, Ectonucleotide Pyrophosphatase/Phosphodiesterase-2, ENPP-2, Lysophospholipase D) is a secreted lysophospholipase D that catalyzes the hydrolysis of lysophosphatidylcholine (LPC) to generate...
Reference: 700620-96
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Urea is the final degradation product of protein and amino acid metabolism. Urea is found in blood and is excreted by the kidney as a component of urine. Besides its role as carrier of waste nitrogen, urea also plays...
Reference: 700640-96
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Lipases perform essential roles in the digestion, transportation, and processing of dietary lipids by controlling the clearance of triglyceride-rich lipoproteins from the circulation. Manipulating lipolysis has...
Reference: 700740-96
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β-Hydroxybutyrate (β-HB; 3-hydroxybutyric acid) is produced in the liver, mainly from the oxidation of fatty acids, and exported to peripheral tissues for use as an energy source. Measurement of β-HB can provide a...
Reference: 700750-96
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Glucose-6-phosphate (G6P) plays a key role in the homeostatic regulation of blood glucose levels by participating in glycolysis, glycogen synthesis, gluconeogenesis, and can be metabolized to NADPH, enabling...
Reference: 700800-96
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Aldehyde dehydrogenases (ALDHs) represent a group of enzymes that oxidize a wide range of endogenous and exogenous aldehydes to their corresponding carboxylic acids, which affect both developmental and toxicological...
Reference: 700870-96
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Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from PUFAs, are unstable...
Reference: 700930-96
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Complex I (NADH oxidase/Co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex I catalyzes the 2 electron oxidation of NADH followed by the...
Reference: 700940-96
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Complex II (succinate dehydrogenase/co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex II catalyzes the oxidation of succinate to...
Reference: 700950-96
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Complex III (CoQ cytochrome c oxidoreductase) is an essential protein for mitochondrial oxidative phosphorylation. Complex III functions as both a gatekeeper for mitochondrial respiration and as a major source of...
Reference: 700990-96
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Complex IV (cytochrome c oxidase) is the terminal electron acceptor in the mitochondrial electron transport chain. Complex IV functions by oxidizing cytochrome c and completing a four-electron reduction of oxygen (O2)...
Reference: 701000-96
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The mitochondrial F1FO ATP synthase, commonly referred to as complex V, is a multi-subunit protein that utilizes energy in the form of a proton gradient generated by the electron transport chain, to phosphorylate ADP...
Reference: 701010-1
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When measuring mitochondrial function or the effects of an unknown on mitochondrial function, it can be difficult to determine a mechanism using whole organisms or tissue. When this is the case, isolated mitochondria...
Reference: 701040-96
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The condensation of the dicarboxylate oxaloacetate and acetyl CoA to the tricarboxylate citrate is catalyzed by citrate synthase. It is within this reaction that carbon molecules (as acetyl CoA) obtained from pyruvate...
Reference: 701080-96
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COX-1 and COX-2 catalyze the production of the PG and TX precursor PGH2. COX-2 is primarily responsible for the biosynthesis of PGs under acute inflammatory conditions. The COX-2 (human recombinant) Inhibitor...
Reference: 701090-96
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COX-1 and COX-2 catalyze the production of the PG and TX precursor PGH2. COX-2 is primarily responsible for the biosynthesis of PGs under acute inflammatory conditions. The COX-1 (ovine) Inhibitor Screening Assay...
Reference: 701220-2
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Cayman's Calcium Assay provides a quick, reliable method for determining total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates. The assay utilizes an optimized...
Reference: 701230-96
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The COX (human) Inhibitor Screening Assay Kit directly measures PGF2α by SnCl2 reduction of COX-derived PGH2 produced in the COX reaction. The prostanoid product is quantified via ELISA using a broadly specific...
Reference: 701310-500
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Cayman’s TMRE Mitochondrial Membrane Potential Assay Kit utilizes tetramethylrhodamine ethyl ester (TMRE), a cell permeable, cationic dye which accumulates in the mitochondrial matrix based on mitochondrial membrane...
Reference: 701320-96
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PAD4 is a guanidino-modifying enzyme that functions as a transcriptional coregulator catalyzing the conversion of specific arginine residues to citrulline. Substrates for PAD4 include histones H2A, H3, and H4. PAD4...
Reference: 701350-96
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Cayman’s α-KG Fluorometric Detection Assay provides a fluorescence-based method for quantifying α-KG in biological samples such as serum, plasma, urine, and tissue. It can also be utilized to determine intracellular...
Reference: 701390-96
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PAD2 is a guanidino-modifying enzyme that catalyzes the conversion of specific arginine residues to citrulline in a calcium-dependent manner. This enzyme has been implicated in several diseases, including rheumatoid...

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