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Citrullinated Histone H3 (Clone 11D3) ELISA Kit

Citrullinated Histone H3 (Clone 11D3) ELISA Kit

Histone H3 is citrullinated by PAD4 at residues R2, R8, and R17 during the processes of epigenetic chromatin remodeling and NET formation. In vitro, CitH3 is released from neutrophils that have been stimulated with calcium ionophore during the process of NETosis, and can be freed from NETs by treatment with nuclease. The measurement of CitH3, either in cell lysates or released during NETosis can be used as a functional readout of pharmacological PAD inhibition.

Citrullinated Histone H3 (Clone 11D3) ELISA Kit

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