EliKine™ Human β-NGF ELISA Kit

EliKine™ Human β-NGF ELISA Kit employs a two-site sandwich ELISA to quantitate β-NGF in samples. An antibody specific for β-NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any β-NGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for β-NGF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of β-NGF bound in the initial step. The color development is stopped and the intensity of the color is measured.