Mouse/Rat PDGF-BB ELISA Kit

KE10034 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse/Rat PDGF-BB ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat PDGF-BB. The assay recognizes mouse/rat PDGF-BB. An antibody specific for mouse/rat PDGF-BB has been pre-coated onto the microwells. The mouse/rat PDGF-BB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse/rat PDGF-BB is added to detect the captured mouse/rat PDGF-BB protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.