Human GM-CSF ELISA Kit

KE00003 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GM-CSF ELISA kit is to be used to detect and quantify protein levels of endogenous GM-CSF. The assay recognizes human GM-CSF. An antibody specific for GM-CSF has been pre-coated onto the microwells. The GM-CSF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for GM-CSF is added to detect the captured GM-CSF protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.