Human IL-28B ELISA Kit

KE00014 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL28B ELISA kit is to be used to detect and quantify protein levels of endogenous IL28B. The assay recognizes human IL28B. An antibody specific for IL28B has been pre-coated onto the microwells. The IL28B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL28B is added to detect the captured IL28B protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.