Human IL-4 ELISA Kit

KE00016 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL4 ELISA kit is to be used to detect and quantify protein levels of endogenous IL4. The assay recognizes human IL4. An antibody specific for IL4 has been pre-coated onto the microwells. The IL4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL4 is added to detect the captured IL4 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.