Human IL-12 p70 ELISA Kit

KE00019 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL12 p70 ELISA kit is to be used to detect and quantify protein levels of endogenous IL12 p70. The assay recognizes human IL12 p70. An antibody specific for IL12 p70 has been pre-coated onto the microwells. The IL12 p70 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL12 p70 is added to detect the captured IL12 p70 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.