Human IFNA1 ELISA Kit

KE00044 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFNA1 ELISA kit is to be used to detect and quantify protein levels of endogenous IFNA1. The assay recognizes human IFNA1. An antibody specific for IFNA1 has been pre-coated onto the microwells. The IFNA1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IFNA1 is added to detect the captured IFNA1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.