Human HER2 ELISA Kit

KE00053 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HER2 ELISA kit is to be used to detect and quantify protein levels of endogenous HER2. The assay recognizes human HER2. An antibody specific for HER2 has been pre-coated onto the microwells. The HER2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for HER2 is added to detect the captured HER2 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.