Human HSP90 ELISA Kit

KE00054 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HSP90 ELISA kit is to be used to detect and quantify protein levels of endogenous HSP90. The assay recognizes human HSP90. An antibody specific for HSP90 has been pre-coated onto the microwells. The HSP90 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for HSP90 is added to detect the captured HSP90 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.