Human CHGA ELISA Kit

KE00057 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CHGA ELISA kit is to be used to detect and quantify protein levels of endogenous CHGA. The assay recognizes human CHGA. An antibody specific for CHGA has been pre-coated onto the microwells. The CHGA protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for CHGA is added to detect the captured CHGA protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.