Human ADAM12 ELISA Kit

KE00058 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ADAM12 ELISA kit is to be used to detect and quantify protein levels of endogenous ADAM12. The assay recognizes human ADAM12. An antibody specific for ADAM12 has been pre-coated onto the microwells. The ADAM12 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for ADAM12 is added to detect the captured ADAM12 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.