Human Albumin ELISA Kit

KE00076 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Albumin ELISA kit is to be used to detect and quantify protein levels of endogenous Albumin. The assay recognizes human Albumin. An antibody specific for Albumin has been pre-coated onto the microwells. The Albumin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for Albumin is added to detect the captured Albumin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.