Human IL-23 ELISA Kit

KE00088 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL23 ELISA kit is to be used to detect and quantify protein levels of endogenous IL23. The assay recognizes human IL23. An antibody specific for IL23 has been pre-coated onto the microwells. The IL23 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL23 is added to detect the captured IL23 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.