Human VEGFR-1 ELISA Kit

KE00113 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The VEGFR-1 ELISA kit is to be used to detect and quantify protein levels of endogenous VEGFR-1. The assay recognizes human VEGFR-1. An antibody specific for VEGFR-1 has been pre-coated onto the microwells. The VEGFR-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for VEGFR-1 is added to detect the captured VEGFR-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.