Human Granzyme B ELISA Kit

KE00121 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Granzyme B ELISA kit is to be used to detect and quantify protein levels of endogenous Granzyme B. The assay recognizes human Granzyme B. An antibody specific for Granzyme B has been pre-coated onto the microwells. The Granzyme B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Granzyme B is added to detect the captured Granzyme B protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.