AuthentiKine™ Human HGF ELISA Kit

KE00168 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HGF ELISA kit is to be used to detect and quantify protein levels of endogenous HGF. The assay recognizes human HGF. An antibody specific for HGF has been pre-coated onto the microwells. The HGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for HGF is added to detect the captured HGF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.