Human S100A8/S100A9 ELISA Kit

KE00177 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The S100A8/S100A9 ELISA kit is to be used to detect and quantify protein levels of endogenous S100A8/S100A9. The assay recognizes human S100A8/S100A9. An antibody specific for S100A8/S100A9 has been pre-coated onto the microwells. The S100A8/S100A9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human S100A8/S100A9 is added to detect the captured human S100A8/S100A9 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm .