Human ENPP-2/Autotaxin ELISA Kit

KE00179 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ENPP-2/Autotaxin ELISA kit is to be used to detect and quantify protein levels of endogenous ENPP-2/Autotaxin. The assay recognizes human ENPP-2/Autotaxin. An antibody specific for ENPP-2/Autotaxin has been pre-coated onto the microwells. The ENPP-2/Autotaxin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human ENPP-2/Autotaxin is added to detect the captured human ENPP-2/Autotaxin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.