AuthentiKine™ Human IL-27 ELISA Kit

KE00182 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL27 ELISA kit is to be used to detect and quantify protein levels of endogenous IL27. The assay recognizes human IL27. An antibody specific for IL27 has been pre-coated onto the microwells. The IL27 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL27 is added to detect the captured IL27 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm .