Human ANGPTL4 ELISA Kit

KE00186 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ANGPTL4 ELISA kit is to be used to detect and quantify protein levels of endogenous ANGPTL4. The assay recognizes human ANGPTL4. An antibody specific for ANGPTL4 has been pre-coated onto the microwells. The ANGPTL4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for ANGPTL4 is added to detect the captured ANGPTL4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .