Human TNFRSF6B/DcR3 ELISA Kit

KE00188 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNFRSF6B/DcR3 ELISA kit is to be used to detect and quantify protein levels of endogenous TNFRSF6B/DcR3. The assay recognizes human TNFRSF6B/DcR3. An antibody specific for TNFRSF6B/DcR3 has been pre-coated onto the microwells. The TNFRSF6B/DcR3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human TNFRSF6B/DcR3 is added to detect the captured human TNFRSF6B/DcR3 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.