Human CCL22/MDC ELISA Kit

KE00189 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL22/MDC ELISA kit is to be used to detect and quantify protein levels of endogenous CCL22/MDC. The assay recognizes human CCL22/MDC. An antibody specific for CCL22/MDC has been pre-coated onto the microwells. The CCL22/MDC protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CCL22/MDC is added to detect the captured human CCL22/MDC protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.