Mouse GM-CSF ELISA Kit

KE10015 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse GM-CSF ELISA kit is to be used to detect and quantify protein levels of endogenous mouse GM-CSF. The assay recognizes mouse GM-CSF. An antibody specific for mouse GM-CSF has been pre-coated onto the microwells. The mouse GM-CSF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse GM-CSF is added to detect the captured mouse GM-CSF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.