Mouse IL-1 alpha ELISA Kit

KE10024 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL1-alpha ELISA kit is to be used to detect and quantify protein levels of endogenous IL1-alpha. The assay recognizes mouse IL1-alpha. An antibody specific for IL1-alpha has been pre-coated onto the microwells. The IL1-alpha protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL1-alpha is added to detect the captured IL1-alpha protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.