Mouse CXCL12/SDF-1 ELISA Kit

KE10049 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CXCL12 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL12. The assay recognizes mouse CXCL12. An antibody specific for CXCL12 has been pre-coated onto the microwells. The CXCL12 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CXCL12 is added to detect the captured mouse CXCL12 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.