Rat Insulin ELISA Kit

KE20008 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Insulin ELISA kit is to be used to detect and quantify protein levels of endogenous Insulin. The assay recognizes Rat Insulin. An antibody specific for Insulin has been pre-coated onto the microwells. The Insulin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for Insulin is added to detect the captured Insulin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm .