Anti-SARS-CoV-2 S-RBD protein Human IgM ELISA Kit

KE30004 is a qualitative measurement of the human IgM for 2019-nCoV S-RBD in serum and plasma. The principle of the kit is indirect ELISA. S-RBD Protein (HEK293 cells expression) has been pre-coated onto microplate well. The samples are added to the well, after incubation the wells are washed and a horseradish peroxidase conjugated Mouse anti-Human IgM is added to each well. Producing an complex “Recombinant Protein–human anti-S-RBD IgM antibody-HRP conjugated second antibody”. after incubation the wells are washed, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.