SARS-CoV-2 N protein ELISA Kit

KE30007 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SARS-CoV-2 N protein ELISA kit is to be used to detect and quantify protein levels of endogenous SARS-CoV-2 N protein. The assay recognizes human SARS-CoV-2 N protein. An antibody specific for SARS-CoV-2 N protein has been pre-coated onto the microwells. The SARS-CoV-2 N protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for SARS-CoV-2 N protein is added to detect the captured SARS-CoV-2 N protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.