KRIBIOLISA™ BNP 32 (Human) (Natrecor) ELISA

Immunoassay for quantitative estimation of BNP32 in human serum, plasma and tissue extracts. BNP32 (Nesiritide/Natrecor) is an endogenous peptide secreted from cardiac ventricles in response to volume increase and pressure overload that acts as an agonist at atrial natriuretic peptide (ANP) receptor A (NRP1).In vitro: BNP 1-32 (10(-6) mol/L) activats cGMP. BNP significantly (P<0.01) inhibited de novo collagen synthesis as assessed by [3H]proline incorporation, whereas zymographic MMP-2 (gelatinase) abundance was significantly (P<0.05) stimulated by BNP between 10(-7) and 10(-6) mol/L. Decreases de novo collagen synthesis and increases MMP gene expression in vitro. In vivo: inhibits the sympathetic and renin-angiotensin-aldosterone systems. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The BNP32 ELISA is a sandwich immunoassay for the determination of BNP32. The antibodies coated on a 96-well plate capture the standard or sample. BNP32 antiserum is subsequently bound to this complex. Biotin is then added followed by SA-HRP (streptavidin-conjugated horseradish peroxidase), which produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific BNP32 bound.