KRIBIOLISA™ Calcitonin (Salmon) (Miacalcic) ELISA

Immunoassay for quantitative estimation of Calcitonin in human serum and plasma. Calcitonin (also known as thyrocalcitonin; Salmon/Miacalcic) is a 32-amino acid linear polypeptide hormone that is produced in humans primarily by the parafollicular cells (also known as C-cells) of the thyroid gland, and in many other animals in the ultimopharyngeal body. It acts to reduce blood calcium (Ca2+), opposing the effects of parathyroid hormone (PTH). Calcitonin injection is used to treat Paget's disease of bone, or high levels of calcium in the blood (hypercalcemia). Calcitonin injection is also used to treat osteoporosis in postmenopausal women. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The Calcitonin ELISA is a sandwich immunoassay for the determination of BNP32. The antibodies coated on a 96-well plate capture the standard or sample. Calcitonin antiserum is subsequently bound to this complex.Biotin is then added followed by SA-HRP (streptavidin-conjugated horseradish peroxidase), which produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific Calcitonin bound.