KRIBIOLISA™ Gonadorelin (LH-RH) (Factrel) ELISA

Immunoassay for quantitative estimation of Gonadorelin in human serum, plasma and tissue extracts. Gonadorelin (Gonadotropin-Releasing Hormone (GnRH), Factrel / HRF / Kryptocur / Lutrelef is a GnRH agonist is used to induce the secretion of the gonadotropins follicle-stimulating hormone and luteinizing hormone from the pituitary gland and to increase sex hormone production by the gonads. It is also used in veterinary medicine. The drug is a pharmaceutical form of endogenous GnRH and is identical to it in chemical structure. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The Gonadorelin ELISA is a competitive immunoassay for the determination of Gonadorelin. The antiserum is captured by antibodies coated on a 96-well plate. A constant concentration of Biotin Concentrate and varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum. Captured Bt-tracer is subsequently bound by SA-HRP (streptavidin-conjugated horseradish peroxidase), which produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of specific Gonadorelin bound.