KRIBIOLISA™ Histrelin (Vantas) ELISA

Immunoassay for quantitative estimation of Histrelin in human serum, plasma and tissue extracts. Histrelin (Supprelin/Vantas) is a gonadotropin releasing hormone (GnRH) agonist that acts as a potentv inhibitor of gonadotropin when administered as an implant that delivers continuous therapeutic doses.Following an initial stimulatory phase with increased circulating levels of luteinizing hormone (LH) and follicle-stimulating hormone (FSH), leading to a transient increase in concentration of gonadal steroids (testosterone and dihydrotestosterone in males), continuous administration of histrelin acetate results in decreased levels of LH and FSH due to a reversible down-regulation of the GnRH receptors in the pituitary gland and desensitization of the pituitary gonadotropes. As the product Supprelin LA (FDA), histrelin is indicated for the treatment of children with central precociouspuberty (CPP). As the product Vantas (FDA), histrelin is indicated for the palliative treatment of advanced prostate cancer. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The Histrelin ELISA is a competitive immunoassay for the determination of Histrelin. The antiserum is captured by antibodies coated on a 96-well plate. A constant concentration of Biotin Concentrate and varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum. Captured Bt-tracer is subsequently bound by SA-HRP (streptavidin-conjugated horseradish peroxidase), which produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of specific Histrelin bound.
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Reference:
KBI5018
Brand:
Product Details
KBI5018

Data sheet

Size
1 x 96 wells
Reactivity
Human
Application
ELISA,Immunoassay
URL - Product
https://krishgen.com/upload/datasheets/KBI5018-Histrelin-ELISA.pdf

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