KRIBIOLISA™ LH (Lutenizing Hormone) Bioassay ELISA

Immunoassay for the Quantification of (hLH) in human Serum Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in response to luteinizing hormone releasing hormone (LH-RH or Gn-RH), released by the hypothalamus. LH, also called interstitial cell-stimulating hormone (ICSH) in men, is a glycoprotein with a molecular weight of approximately 30,000 Daltons. It is composed of two non-covalently associated dissimilar amino acid chains, alpha and beta. A lack of secretion by the anterior pituitary may cause lower LH levels. As may be expected, low levels may result in infertility in both males and females. Low levels of LH may also be due to the decreased secretion of GnRH by the hypothalamus. Low LH values may therefore indicate some dysfunction of the pituitary or hypothalamus, but the actual source of the problem must be confirmed by other tests.10 About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to LH are pre-coated onto microwells. Samples and standards are pipetted into microwells and anti-hLH antibodies linked to HRP is added to the wells. LH present in the sample and standards will bind to the plate and HRP Conjugate simultaneously. Washing is done to remove the unbound samples, standards and HRP conjugate. The ready to use substrate solution (TMB) is added to microwells and color develops directly proportional to the amount of LH in the standards and sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.