KRIBIOLISA™ Gentamicin ELISA

Enzyme Immunoassay for the Quantitative Estimation of Gentamicin in cell culture supernatants and other biological preparations. Gentamycin residue in the production of biological samples may lead to severe allergic reactions in certain groups. Thus it is strictly controlled in many countries in the world and the analyte needs to be measured in biological preparations before release. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The method employs sandwich ELISA technique. Monoclonal antibodies to Gentamicin are pre-coated onto microwells. Samples and standards are pipetted into microwells and Gentamicin present in the sample are bound by the antibodies competing with Gentamicin labeled HRP. Post incubation a complex is formed. After washing the microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Gentamicin in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.