KRIBIOLISA™ Protein A ELISA (natural & structurally conserved recombinant Protein A specific)

Enzyme Immunoassay for the quantitative determination of Protein A in biological solutions. A variety of antibodies are used as therapeutics agent as well as in pharmaceuticals. These antibodies are generally purified through protein A column chromatography. This method of purification has the potential with contamination of protein A, which might get leached out of the column and co-eluted with purified antibody. This may result in adverse toxic or immunological reactions that ultimately affect the efficacy of the product antibody. The highly sensitive Protein A ELISA is a quantitative, simple and will aid in monitoring the purification process as well as screening product prior to its use. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year This assay is based on the principle of Sandwich ELISA. Samples containing Protein A are reacted with anti- Protein A:HRP antibody simultaneously in the microtiter wells already coated with affinity purified capture anti- Protein A antibody. This immunological reaction results in formation of a sandwich complex of solid phase antibody-Protein-enzyme labeled antibody. The wells are washed to remove any unbound reactants as per the wash procedure (see Assay procedure section mentioned below). TMB substrate is then added. Color develops proportionally to the amount of Protein A in the samples. Color development is stopped by addition of stop solution. Absorbance is measured at 450 nm