T7 RNAi Transcription Kit

T7 RNA ploymerase can recognize DNA templates with T7 promoters, and use four NTPs as substrates to synthesize RNA by in vitro transcription. The T7 RNAi Transcription Kit is an optimized version designed for the transcription of double-stranded RNA based on the T7 High Yield RNA Transcription Kit. It can be used to transcribe 21 bp siRNA and long fragment dsRNA. The purified transcript can be used in RNAi experiments mediated by cationic liposomes, calcium phosphate co-precipitation, electroporation, DEAE-dextran, and microinjection. In general, one reaction can produce 20-80 µg of RNA.Application: Can transcribe siRNA and long dsRNA; The yield is as high as 20-80 μg; RNA magnetic beads can quickly and efficiently purify transcription products