2× EpiArt HS Taq Master Mix

This product contains EpiArtTM HS Taq DNA Polymerase, dNTP and optimized buffer system, which is suitable for the PCR reaction of bisulfite conversion processing DNA. Compared with ordinary DNA, the DNA damage after bisulfite conversion is serious, which will affect the PCR reaction performance. This product uses a hot-start EpiArtTM HS Taq DNA Polymerase modified by enzyme engineering and an optimized buffer system. Compared with ordinary PCR reagents, this product has better amplification efficiency, sensitivity and specificity for the bisulfite-converted DNA . The pre-prepared 2 × Master Mix only needs to add primers and templates to perform amplification when used in PCR reactions, which reduces pipetting operations and improves detection throughput and results reproducibility. EM202 contains blue dye, which can be directly used for electrophoresis after the reaction, which is convenient to use. This product can be used with EpiArtTM DNA Methylation Bisulfite Kit (Vazyme #EM101). The PCR product has A at the 3'end, which can be directly cloned into the T vector. It is suitable for ClonExpress® rapid cloning kit (Vazyme #C112/C113/C115) ) And 5 min TA/Blunt-Zero Cloning Kit (Vazyme #C601).Application: (1) Optimized buffer system, suitable for PCR reaction of bisulfite conversion processing DNA (2) Compared with ordinary PCR reagents, it has better amplification efficiency, sensitivity and specificity for DNA after bisulfite conversion (3) EM202 contains blue dye, which can be directly electrophoresed after the reaction, which is convenient to use