2× EpiArt HS Taq Master Mix (Dye Plus)

This product contains EpiArtTM HS Taq DNA Polymerase, dNTP and optimized buffer system, which is suitable for the PCR reaction of bisulfite conversion processing DNA. Compared with ordinary DNA, the DNA damage after bisulfite conversion is serious, which will affect the PCR reaction performance. This product uses a hot-start EpiArtTM HS Taq DNA Polymerase modified by enzyme engineering and an optimized buffer system. Compared with ordinary PCR reagents, this product has better amplification efficiency, sensitivity and specificity for the bisulfite-converted DNA . The pre-prepared 2 × Master Mix only needs to add primers and templates to perform amplification when used in PCR reactions, which reduces pipetting operations and improves detection throughput and results reproducibility. EM202 contains blue dye, which can be directly used for electrophoresis after the reaction, which is convenient to use. This product can be used with EpiArtTM DNA Methylation Bisulfite Kit (Vazyme #EM101). The PCR product has A at the 3'end, which can be directly cloned into the T vector. It is suitable for ClonExpress® rapid cloning kit (Vazyme #C112/C113/C115) ) And 5 min TA/Blunt-Zero Cloning Kit (Vazyme #C601).Application: High cost performance; Short delivery time; It has exactly the same usage as AMPure XP Beads, no need to re-explore the conditions; The recovery rate, library size and AMPure XP Beads are highly consistent; It is suitable for DNA and RNA library building, and has good compatibility with all brands of library building reagents; Strict batch quality control, better price, shorter delivery time