Phanta® Max Super-Fidelity DNA Polymerase

Phanta® Max Super-Fidelity DNA Polymerase is an upgraded version of Phanta® Super-Fidelity DNA Polymerase. Compared with the previous generation of products, Phanta® Max has added unique elongation factors, specific promotion factors and plateau release inhibitors. It has greatly improved the ability of long fragment amplification, amplification specificity and amplification yield. Using simple templates such as lambda DNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using genomic DNA, etc. For complex templates, Phanta® Max can amplify fragments up to 20 kb; using cDNA templates, Phanta® Max can effectively amplify fragments up to 10 kb. Its mismatch rate is 1/53 of that of ordinary Taq enzyme, and it is Pfu enzyme 1/6. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max is added to inhibit at room temperature Its two monoclonal antibodies with 5'→3' polymerase activity and 3'→5' exonuclease activity can perform highly specific hot-start PCR. The amplified products are blunt-ended and are suitable for ClonExpress® fast Rapid cloning kit (C112/C113/C114). Application: Super fidelity: the fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. Every 300,000 bases amplified, with less than 5 mismatches; Faster amplification: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments; Longer amplification: the effective amplification length of simple templates such as plasmid and λ DNA can reach 40 kb, the effective amplification length of cDNA can reach 10 kb, and the effective amplification length of genome can reach 20 kb; Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.